Redesign of the monomer–monomer interface of Cre recombinase yields an obligate heterotetrameric complex
نویسندگان
چکیده
منابع مشابه
Redesign of the monomer–monomer interface of Cre recombinase yields an obligate heterotetrameric complex
Cre recombinase catalyzes the cleavage and religation of DNA at loxP sites. The enzyme is a homotetramer in its functional state, and the symmetry of the protein complex enforces a pseudo-palindromic symmetry upon the loxP sequence. The Cre-lox system is a powerful tool for many researchers. However, broader application of the system is limited by the fixed sequence preferences of Cre, which ar...
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چکیده ندارد.
15 صفحه اولMegakaryocyte-restricted expression of the Cre recombinase.
doi:10.1160/TH11-03-0150 Thromb Haemost 2011; 106: 554 Dear Sirs, In the recently published work from Adam Nowakowski et al. “Megakaryocyte gene targeting mediated by restricted expression of recombinase Cre” (Thromb Haemost 2011; 105.1) the authors present a transgenic mouse strain that allows Cre expression under the control of the alphaIIb promoter (1). Nowakowski et al. (1) suggest that thi...
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Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery vectors. The specificity of recombinases can be altered either with directed evolution or via fusions to modular DNA-binding domains. Unfortunat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2015
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkv901